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Triple Helix Forming Oligonucleotides

Triple Helix Forming Oligonucleotides PDF Author: Claude Malvy
Publisher: Springer Science & Business Media
ISBN: 1461551773
Category : Science
Languages : en
Pages : 297

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Book Description
Sequence-specific DNA binding ligands, amongst which triple helix forming oligonucleotides are the most efficient as yet, represent promising tools in a number of fields. One of their most promising applications is as antiviral tools: they can specifically target a viral gene, even if it is integrated into the host genome, and be used to specifically inactivate the viral gene or even destroy the cells harboring this gene. However, from science fiction to science there remains a gap; and we are at the moment on the threshold of this fascinating field. Triple Helix Forming Oligonucleotides considers the different aspects of the design and improvement, current or future, of these molecules and their structural analysis, as well as their applications, with special emphasis on the attempts to obtain biological effects of these potentially important tools. What emerges is that the current state of the research is encouraging, and that these molecules are already useful in some biotechnology applications.

Triple Helix Forming Oligonucleotides

Triple Helix Forming Oligonucleotides PDF Author: Claude Malvy
Publisher: Springer Science & Business Media
ISBN: 1461551773
Category : Science
Languages : en
Pages : 297

View

Book Description
Sequence-specific DNA binding ligands, amongst which triple helix forming oligonucleotides are the most efficient as yet, represent promising tools in a number of fields. One of their most promising applications is as antiviral tools: they can specifically target a viral gene, even if it is integrated into the host genome, and be used to specifically inactivate the viral gene or even destroy the cells harboring this gene. However, from science fiction to science there remains a gap; and we are at the moment on the threshold of this fascinating field. Triple Helix Forming Oligonucleotides considers the different aspects of the design and improvement, current or future, of these molecules and their structural analysis, as well as their applications, with special emphasis on the attempts to obtain biological effects of these potentially important tools. What emerges is that the current state of the research is encouraging, and that these molecules are already useful in some biotechnology applications.

A System for the Intracellular Generation of Triple Helix-forming Oligonucleotides (TFOs) and the Sequence-specific Inhibition of Human MCP-1 Gene Expression

A System for the Intracellular Generation of Triple Helix-forming Oligonucleotides (TFOs) and the Sequence-specific Inhibition of Human MCP-1 Gene Expression PDF Author: Kordula Kautz
Publisher:
ISBN:
Category :
Languages : en
Pages : 282

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Mutagenic Properties of Psoralen-modified Triple Helix Forming Oligonucleotides

Mutagenic Properties of Psoralen-modified Triple Helix Forming Oligonucleotides PDF Author: Zoltán Sándor
Publisher:
ISBN:
Category : Oligonucleotides
Languages : en
Pages : 69

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Triple-Helical Nucleic Acids

Triple-Helical Nucleic Acids PDF Author: Valery N. Soyfer
Publisher: Springer Science & Business Media
ISBN: 1461239729
Category : Science
Languages : en
Pages : 360

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Book Description
The ability of DNA to exist in configurations other than its classical double-stranded form has been known for many years. There has been a spectacular recent surge of interest in these forms, notably in the three-stranded or triple-helical form. Triplex-like nucleic acids are now known to exist in vivo, and may well participate in significant biological processes. Interest in triple-helical nucleic acids has been greatly stimulated by their potential exploitation to control gene expression, serve as tools in genome mapping strategies, etc. The authors have written an encyclopedic introduction to nucleic acid triplexes based on many years of familiarity with the topic. The book includes information on chemistry, conformation, physical properties, applications, and hypotheses about the biological role of triplexes. It pays particular attention to the different methods for investigating these molecules, a feature which will be welcomed by those new to the field.

Regulation of Gene Expression in Vivo Mediated by Triple Helix- Forming Oligonucleotides

Regulation of Gene Expression in Vivo Mediated by Triple Helix- Forming Oligonucleotides PDF Author: Cheryl Anne Hobbs
Publisher:
ISBN:
Category :
Languages : en
Pages : 230

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Applied Antisense Oligonucleotide Technology

Applied Antisense Oligonucleotide Technology PDF Author: Cy A. Stein
Publisher: Wiley-Liss
ISBN:
Category : Medical
Languages : en
Pages : 540

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Book Description
Applied Antisense Oligonucleotide Technology provides the basic concepts as well as the practical concerns associated with the use of antisense oligonucleotides to modify gene expression. Chapters include oligonucleotide chemistry, DNA triplex formation, delivery mechanisms, pharmocokinetics, toxicity, oligonucleotides.

The Design of Oligonucleotides Which Attack Specific Gene Targets

The Design of Oligonucleotides Which Attack Specific Gene Targets PDF Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 6

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Book Description
Triple Helix Design Principles. The first research priority of the program of Navy support was to refine our understanding of triple helix forming oligonucleotides (TFOs). Binding affinity and strand orientation of triplex forming oligonucleotides were measured as a function of base composition. Based upon that work, which was published in Biochemistry (ref. 3), we showed that triple helices containing GGC and TAT triplets were stable at physiological pH and prefer to bind with an antiparallel strand orientation. This study and the accompanying patent application provided the first evidence that TFOs can bind in a site selective fashion at physiological pH and the first explicit evidence for a new (antiparallel) class of triple helix. This work also served as the basis for the filing of a continuation in part to a patent application, filed 12/89. DNA Recognition, Triplet helix formation.

Recognition of Double Helical DNA by Purine Oligonucleotides Via Triple Helix Formation

Recognition of Double Helical DNA by Purine Oligonucleotides Via Triple Helix Formation PDF Author: Peter A. Beal
Publisher:
ISBN:
Category :
Languages : en
Pages : 382

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Oligonucleotides as Therapeutic Agents

Oligonucleotides as Therapeutic Agents PDF Author: Derek J. Chadwick
Publisher: John Wiley & Sons
ISBN: 0470515406
Category : Science
Languages : en
Pages : 260

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Book Description
The use of oligonucleotides as therapeutic agents rests upon their ability to interfere, in a sequence-specific manner, with the fundamental machinery of protein synthesis either by binding to the mRNAs transcribed from a gene or by binding directly to a target gene. This approach can be used not only for inhibition of the synthesis of host proteins but also of those required by invading pathogens. Potential therapeutic applications are enormous, ranging over hypertension, cardiovascular disease, autoimmune disease, vital and other parasitic infections (especially HIV), and cancer. This book discusses the chemistry and pharmacokinetics of oligonucleotides and their analogues, and surveys the results of structure-activity studies and current clinical trials. It also critically reviews the problems with antisense therapy, such as the enzymatic destruction of oligonucleotides, the doses required for a therapeutic response, the difficulty in directing oligonucleotides to particular target tissues and cells, the need for parenteral administration, and doubts concerning the mechanism of action (especially problems associated with non-specific binding to proteins) and long-term effects.

Oligonucleotide Synthesis

Oligonucleotide Synthesis PDF Author: Piet Herdewijn
Publisher: Springer Science & Business Media
ISBN: 1592598234
Category : Science
Languages : en
Pages : 435

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Book Description
Nucleic acids chemistry has progressed rapidly in the last 10 years with the introduction of many new techniques and the development of many novel therapeutic entities. In Oligonucleotide Synthesis: Methods and Applications, laboratory experts describe in step-by-step detail the powerful new techniques they have developed for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are the synthesis of phosphorothioates via new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, triple helix formation, and a novel two-step process that yields a high purity DNA that is less costly. Novel applications include the use of molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules to DNA, nucleic acid libraries, optimization of RNA synthesis for siRNA applications, and the use of small interference RNA (siRNA) as an inhibitor of gene expression. The protocols follow the successful Methods in Molecular Biology"!series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Oligonucleotide Synthesis: Methods and Applications offers today's investigators not only insight into important new biotechnologies, but also a full range of the detailed protocols needed to work successfully in DNA synthesis and its applications today